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ISO 5815-2:2003

Water quality — Determination of biochemical oxygen demand after n days (BODn) — Part 2: Method for undiluted samples

Edition date: 2003-03-31
In Force
Available languages: English, French
Summary:

ISO 5815-2:2003 specifies determination of the biochemical oxygen demand of waters of undiluted samples. It is applicable to all waters having biochemical oxygen demands greater than or equal to 0,5 mg/l of oxygen (the limit of determination) and not exceeding 6 mg/l of oxygen.

The results obtained are the product of a combination of biochemical and chemical reactions. They do not have the rigorous and unambiguous character of those resulting from, for example, a single, well-defined, chemical process. Nevertheless, they provide an indication from which the quality of waters can be estimated.

The test can be influenced by the presence of various substances. Those which are toxic to microorganisms, for example bactericides, toxic metals or free chlorine, inhibit biochemical oxidation. The presence of algae or nitrifying microorganisms can produce artificially high results. In these situations a modification of the method may be necessary.

Annex A describes alternative incubation periods.

Annex B describes procedures for modification of the method by addition of seeding material, salts, inhibition of nitrification by allylthiourea (ATU) addition, neutralization, homogenization and/or filtration. These modifications may be found necessary for specific evaluations of the water quality of receiving waters.

Annex C provides precision data.



L'ISO 5815-2:2003 spécifie une méthode de détermination de la demande biochimique en oxygène (DBO) dans les eaux pour des échantillons non dilués. La méthode est applicable à tous les types d'eau dont la demande biochimique en oxygène est supérieure ou égale à la limite de détermination de 0,5 mg/l d'oxygène et ne dépasse pas 6 mg/l d'oxygène.

Les résultats obtenus sont le produit d'une combinaison de réactions biochimiques et chimiques. Ils ne possèdent pas le caractère exact et rigoureux tel que, par exemple, celui d'un processus chimique unique bien défini. Ils fournissent cependant une indication qui permet d'estimer la qualité des eaux.

L'analyse peut être influencée par la présence de substances diverses. Celles qui sont toxiques pour les micro-organismes telles que, par exemple, les bactéricides, les métaux toxiques ou le chlore libre, inhibent l'oxydation biochimique. La présence d'algues ou de micro-organismes nitrificateurs peut entraîner des résultats artificiellement élevés. Dans ces conditions, une modification de la méthode peut être nécessaire.

L'annexe A décrit des périodes d'incubation alternatives.

L'annexe B décrit des procédures pour la modification de la méthode par l'apport de substances d'ensemencement, de sels, d'allylthiourée (ATU) pour l'inhibition de la nitrification, par neutralisation, par homogénéisation et/ou par filtration. Ces modifications peuvent être nécessaires pour des évaluations spécifiques de la qualité de l'eau des milieux récepteurs.

L'Annexe C fournit des données de fidélité.
ICS: 13.060.50-Examination of water for chemical substances
CTN: ISO/TC 147/SC 2 - 52846

Standards Cancellations

Anula a ISO 5815:1989

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