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CEN/TS 16707:2014

Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Polymerase chain reaction (PCR) based screening strategies

Edition date: 2024-09-17
In Force
Available languages: German, English, French
Summary: Diese Technische Spezifikation beschreibt Screening-Strategien für den Nachweis von gentechnisch modifizierter (gm) oder gentechnisch veränderter (gv) DNA in Lebensmitteln mittels PCR Verfahren. Die Strategien wurden für Lebensmittelmatrices erarbeitet, können jedoch auch auf andere Matrices angewendet werden (z. B. Futtermittel, Saatgut und Proben von Feldpflanzen). Der Nachweis von gv-DNA beruht auf PCR Verfahren, die einzelne Segmente transgener DNA Sequenzen (genetische Elemente, genetische Konstrukte oder Insertionsorte von Transgenen) nachweisen. Verschiedene Kombinationen dieser PCR Verfahren werden in die Screening-Strategien einbezogen. Die Verfahren werden gleichzeitig oder hierarchisch angewendet. Die allgemeine Analysestrategie beruht auf dem Matrix-Ansatz. Beispiele für die Implementierung und Anwendung dieses Ansatzes sind beschrieben. Um zuverlässige Analyseergebnisse sicherzustellen, enthält das vorliegende Dokument außerdem Leitlinien für die Validierung der Leistungskriterien von qualitativen PCR Verfahren, die bei Screening-Ansätzen angewendet werden.

This Technical Specification describes screening strategies for the detection of genetically modified (GM) DNA in food products by means of PCR methods. The strategies have been established for food matrices, but it can also be applied to other matrices (e.g. feed, seed and samples from field grown plants). Detection of GM DNA is based on PCR methods targeting segments of transgenic DNA sequences (genetic elements, genetic constructs or insertion sites of transgenes). Various combinations of these PCR methods are involved in screening strategies. The methods are applied simultaneously or hierarchically. The general strategy is based on the matrix approach. Examples for the implementation and application of this approach are described. In order to ensure reliable analytical results, the document also provides guidelines for the validation of the performance criteria of qualitative PCR methods applied in screening approaches.

La présente Spécification technique décrit des stratégies de criblage pour la détection d’ADN génétiquement modifié (GM) dans les produits alimentaires au moyen des méthodes de PCR. Ces stratégies ont été élaborées pour les matrices alimentaires mais elles peuvent également être appliquées à d’autres matrices (par exemple aliments pour animaux, graines et échantillons issus de plantes cultivées en plein champ). La détection d’ADN GM est basée sur des méthodes de PCR ciblant des segments de séquences d’ADN transgéniques (éléments génétiques, construits génétiques ou sites d’insertion de transgènes). Les stratégies de criblage font intervenir différentes combinaisons de ces méthodes de PCR. Ces méthodes sont appliquées simultanément ou de façon hiérarchique. La stratégie générale repose sur l’approche matricielle. Des exemples de mise en oeuvre et d’application de cette approche sont décrits. Pour garantir des résultats analytiques fiables, le document fournit également des lignes directrices pour la validation des critères de performance des méthodes de PCR qualitative utilisées dans les approches de criblage.
ICS: 67.050-General methods of tests and analysis for food products
CTN: CEN/TC 275 - 6256

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