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CEN ISO/TS 13136:2012

Microbiology of food and animal feed - Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens - Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination of O157, O111, O26, O103 and O145 serogroups (ISO/TS 13136:2012)

Edition date: 2012-11-15
In Force
Available languages: English, French
Summary: ISO/TR 13136:2012 describes the identification of Shiga toxin-producing Escherichia coli (STEC) by means of the detection of the following genes: a) the major virulence genes of STEC, stx and eae; b) the genes associated with the serogroups O157, O111, O26, O103, and O145. In any case, when one or both of the stx genes is/are detected, the isolation of the strain is attempted. The isolation of STEC from samples positive for the presence of the genes specifying the serogroups in the scope of this method can be facilitated by using serogroup-specific enrichment techniques (e.g. immunomagnetic separation, IMS). The protocol uses real-time PCR as the reference technology for detection of the virulence and serogroup-associated genes. ISO/TR 13136:2012 is applicable to: 1) products intended for human consumption and the feeding of animals; 2) environmental samples in the area of food production and food handling; 3) environmental samples in the area of primary production.

Diese Technische Spezifikation beschreibt die Identifizierung von Shiga Toxin bildenden Escherichia coli (STEC) mit Hilfe des Nachweises folgender Gene: a) der wichtigsten Virulenzgene von STEC, stx und eae (Literaturhinweise [2], [3]); b) der mit den Serogruppen O157, O111, O26, O103 und O145 assoziierten Gene (Literaturhinweise [3], [4]). In jedem Fall, wenn eines oder beide der stx Gene nachgewiesen wird/werden, wird versucht, den Stamm zu isolieren. Die Isolierung von STEC aus Proben, die positiv hinsichtlich der Anwesenheit von Genen solcher Serogruppen sind, die im Anwendungsbereich dieses Verfahrens liegen, kann durch die Anwendung serogruppenspezifischer Anreicherungsverfahren (z. B. immunomagnetische Separation, IMS) erleichtert werden. In dem beschriebenen Verfahren wird die Real-time-PCR als das Referenzverfahren zum Nachweis der Virulenzgene und der mit bestimmten Serogruppen-assoziierten Gene angewendet. Die vorliegende Technische Spezifikation ist anwendbar auf: 1) Erzeugnisse, die für den menschlichen Verzehr und als Futtermittel vorgesehen sind; 2) Umgebungsproben im Bereich der Herstellung und Handhabung von Lebensmitteln; 3) Umgebungsproben im Bereich der Primärproduktion.

L'ISO/TS 13136:2012 décrit une méthode horizontale pour la détection: a)des principaux gènes de virulence des STEC; b) des gènes associés aux sérogroupes O157, O111, O26, O103 et O145. Dans le cas de la détection de ces gènes, une procédure d'isolement de souches est mise en place afin de confirmer la présence simultanée des gènes dans une seule et même cellule bactérienne vivante. L'ISO/TS 13136:2012 repose sur l'utilisation de la PCR en temps réel comme technologie de référence pour la détection des gènes de virulence et des gènes associés aux sérogroupes. Un protocole de PCR en temps réel est par conséquent décrit en détail. L'ISO/TS 13136:2012 s'applique: 1) aux produits destinés à être consommés par l'Homme et aux aliments pour animaux; 2) aux échantillons environnementaux dans la zone de production et de manipulation des aliments; 3) aux échantillons environnementaux dans la zone de production primaire.
ICS: 07.100.30-Food microbiology
CTN: CEN/TC 463 - 2648347

Standards Cancellations

Es anulada por prEN ISO 13136-1

Es anulada por prEN ISO 13136-2

Otras Relaciones

Acuerdo de Viena ISO/TS 13136:2012

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