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NF EN 17294

Animal feeding stuffs - Methods of sampling and analysis - Determination of organic acids by Ion Chromatography with Conductivity Detection (IC-CD)

Edition date: 2019-08-07
In Force
Available languages: English, French
Summary: Domaine d'applicationLe présent document spécifie une méthode de dosage des acides organiques dans les aliments pour animaux par chromatographie ionique avec détection conductimétrique (CI-DC) .La méthode est destinée à permettre le dosage de l'acide formique, de l'acide lactique, de l'acide propionique, de l'acide citrique, de l'acide fumarique et de l'acide malique présents comme substances actives dans les additifs pour l'alimentation animale, les prémélanges, les matières premières pour aliments des animaux, les aliments composés pour animaux, ainsi que l'eau et l'acide acétique de manière limitée dans les mêmes matrices. Cette méthode détermine la concentration totale extractible des acides organiques susmentionnés et de leurs sels.Il est recommandé à l'utilisateur de la présente norme de déterminer le domaine de mesure de la méthode pour chaque acide organique. La limite inférieure du domaine de mesure dépend de la matrice et des interférences rencontrées. Il est recommandé de pouvoir atteindre un domaine de mesure compris entre 10 mg/l et 100 mg/l.La méthode a été soumise à essai avec succès lors d'une étude interlaboratoires à des concentrations allant de 0,02 pour cent à 27 pour cent pour les acides organiques susmentionnés.La détermination simultanée d'une concentration élevée d'acide lactique et d'une faible concentration d'acide acétique présente des limites. Si le rapport de la concentration d'acide lactique à la concentration d'acide acétique dépasse un facteur de 20, le dosage de l'acide acétique n'est pas garanti.En s'appuyant sur le domaine de mesure mentionné, le poids de l'échantillon et le volume d'extraction, il est possible d'atteindre les limites de quantification (LQ) , telles que calculées (Tableau 1) .- Limites de quantification (LQ) Acide organiqueLQ [mg/kg]Acide formique200Acide lactique200Acide propionique200Acide citrique200Acide fumarique200Acide acétique200Acide malique200
Keywords: ANIMAL FEED|ANIMAL FEEDING PRODUCTS|FOOD ADDITIVES|COMPOUND FEED|WATER|CHEMICAL ANALYSIS|DETERMINATION OF CONTENT|ORGANIC ACIDS|CITRIC ACID|LACTIC ACID|ACETIC ACID|MALIC ACID|FORMIC ACID|SAMPLING|EXTRACTION|CHROMATOGRAPHY|DETECTION|CONDUCTIMETRIC METHODS|CALIBRATION|COMPUTATION|FIDELITY
ICS: 65.120-Animal feeding stuffs, 67.220.20-Food additives
CTN:

International Equivalence

Identic EN 17294:2019

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