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NF EN 17256

Animal feeding stuffs : methods of sampling and analysis - Determination of ergot alkaloids and tropane alkaloids in feed materials and compound feeds by LC-MS/MS

Edition date: 2019-10-09
In Force
Available languages: English, French
Summary: Domaine d'applicationLe présent document décrit une méthode de détermination de la teneur en alcaloïdes de l'ergot et en alcaloïdes tropaniques dans les céréales non transformées et les aliments composés à base de céréales par chromatographie liquide à haute performance couplée à une spectrométrie de masse en tandem (CL-SM/SM) .Le présent document a été validé lors d'un essai interlaboratoires dans les matrices suivantes : seigle, orge, blé, aliments complets pour bovins, porcins et volailles. La validation dans le sarrasin a donné des résultats acceptables, mais le coefficient de variation de la reproductibilité était plus élevé pour la plupart des analytes par rapport aux autres matrices. Ceci peut être dû à la matrice. La gamme validée de la méthode est d'environ 10 micron g/kg à 250 micron g/kg pour les alcaloïdes. Il est possible de déterminer des concentrations supérieures à 250 micron g/kg en appliquant un niveau de dopage et une dilution plus élevés de l'extrait d'échantillon, mais ceci n'a pas été validé lors de l'essai interlaboratoires.Le présent document est applicable à la détermination, par ajout dosé en un point dans l'échantillon :de la teneur en ergocornine dans la gamme d'essai de 12 micron g/kg à 221 micron g/kg ;de la teneur en ergocorninine dans la gamme d'essai de 9 micron g/kg à 196 micron g/kg ;de la teneur en ergocristine dans la gamme d'essai de 14 micron g/kg à 312 micron g/kg ;de la teneur en ergocristinine dans la gamme d'essai de 12 micron g/kg à 258 micron g/kg ;de la teneur en a-ergocryptine dans la gamme d'essai de 10 micron g/kg à 184 micron g/kg ;de la teneur totale en a-ergocryptinine et en ß-ergocryptinine dans la gamme d'essai de 8 micron g/kg à 171 micron g/kg ;de la teneur en ergométrine dans la gamme d'essai de 12 micron g/kg à 174 micron g/kg ;de la teneur en ergométrinine dans la gamme d'essai de 3 micron g/kg à 172 micron g/kg ;de la teneur en ergosine dans la gamme d'essai de 12 micron g/kg à 226 micron g/kg ;de la teneur en ergosinine dans la gamme d'essai de 9 micron g/kg à 273 micron g/kg ;de la teneur en ergotamine dans la gamme d'essai de 11 micron g/kg à 443 micron g/kg ;de la teneur en ergotaminine dans la gamme d'essai de 10 micron g/kg à 273 micron g/kg ;de la teneur en atropine dans la gamme d'essai de 16 micron g/kg à 252 micron g/kg ;de la teneur en scopolamine dans la gamme d'essai de 15 micron g/kg à 246 micron g/kg.
Keywords: ANIMAL FEED|RAW MATERIALS|ANIMAL FEEDING PRODUCTS|GRAIN CROPS|LABORATORY TESTS|DETERMINATION|ALKALOIDS|MYCOTOXINS|PROCEDURE|CHROMATOGRAPHY|MASS SPECTROMETERS|SAMPLES|QUALITY CONTROL|QUANTITATIVE ANALYSIS|FIDELITY|REPRODUCIBILITY|TABLES (DATA)|TEST RESULTS
ICS: 71.040.40-Chemical analysis, 65.120-Animal feeding stuffs
CTN:

International Equivalence

Identic EN 17256:2019

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