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DIN EN 17882:2022-08
Food authenticity - DNA barcoding of meat and meat products derived from mammalia and poultry using defined mitochondrial cytochrome b and cytochrome c oxidase I gene segments; German and English version prEN 17882:2022 / Note: Date of issue 2022-07-08
| Fecha edición: |
2024-10-01
Anulada
|
|---|---|
| Fecha cancelación: | 2024-10-01 |
| Idiomas disponibles: | Alemán, Inglés |
| Resumen: | This document describes a procedure for the identification of meat and meat products derived from mammalia and poultry to the level of genus or species. The identification of meat species is carried out by PCR amplification of either a segment of the mitochondrial cytochrome b gene (cytb) or the cytochrome c oxidase I gene (cox1, syn COI), or both, followed by sequencing of the PCR products and subsequent sequence comparison with entries in databases. The methodology allows the identification of a large number of frequently used as well as exotic meat species in foodstuffs. The decision whether the cytb or cox1 gene segment or both are used for meat identification depends on the declared meat species, the applicability of the PCR method for the meat species and the availability of comparative sequences in the public databases. This method has been successfully validated on raw meat, however, laboratory experience is available that it can also be applied to processed meat products. This document is usually unsuitable for the analysis of highly processed foods with highly degraded DNA where the fragment lengths are not sufficient for amplification of the targets. Furthermore, it is not applicable for complex meat products containing mixtures of two or more meat species. |
| Keywords: | Analysis|Bar code|Birds|Definitions|DNA|Extraction|Food products|Food protection|Food testing|Fraud|Genes|Genetics|Identification|Mammalia|Marking|Meat|Meat products|Molecular biological methods|PCR|Safety|Sample preparation|Segments|Sequencing|Species (biology)|Traceability |
| ICS: | 67.020 - Procesos en la industria alimentaria, 67.120.10 - Carne y productos cárnicos, 07.080 - Biología. Botánica. Zoología |
| CTN: | |
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Reemplazo Normas |
Es reemplazada por DIN EN 17882:2024-10 |










