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CEN ISO/TS 6579-2:2012

Microbiology of food and animal feed - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 2: Enumeration by a miniaturized most probable number technique (ISO/TS 6579-2:2012)

Edition date: 2012-11-01
In Force
Available languages: English, French
Summary: ISO/TS 6579-2 gives a method for the enumeration of Salmonella spp. present in: products intended for human consumption and for the feeding of animals; environmental samples in the area of food production and food handling; animal faeces; and environmental samples from the primary production stage by calculation of the most probable number (MPN). The method is based on miniaturization of the dilution, pre-enrichment and selective enrichment steps. The selective enrichment medium, modified semi-solid Rappaport?Vassiliadis (MSRV), is intended for the detection of motile salmonellae and is not appropriate for the detection of non-motile salmonellae. It is possible that the method is less appropriate to enumerate Salmonella ser. Typhi and Salmonella ser. Paratyphi. The method is not appropriate for the enumeration of Salmonella spp. in (very) low contaminated samples (<1 cfu/g).

L'ISO/TS 6579-2:2012 fournit une méthode de dénombrement de Salmonella spp. présentes dans: les produits destinés à la consommation humaine ou à l'alimentation animale; les échantillons environnementaux dans le domaine de la production et de la manutention de produits alimentaires; les matières fécales des animaux; les échantillons environnementaux au stade de la production primaire; par le calcul du nombre le plus probable (NPP). La méthode est fondée sur une miniaturisation des étapes de dilution, de préenrichissement et d'enrichissement sélectif. Le milieu d'enrichissement sélectif, le milieu semi-solide de Rappaport-Vassiliadis modifié (MSRV), est conçu pour la recherche des Salmonella mobiles et n'est pas adapté à la recherche des Salmonella immobiles. Il est possible que la méthode soit moins appropriée au dénombrement de Salmonella ser. Typhi et Salmonella ser. Paratyphi. La méthode n'est pas appropriée au dénombrement de Salmonella spp. présentes en (très) faibles concentrations dans des échantillons contaminés (< 1 ufc/g).

Dieser Teil von ISO 6579 legt ein Verfahren zur Zählung von Salmonella spp. in - Produkten, die für den menschlichen Verzehr oder als Futtermittel bestimmt sind; - Umgebungsproben im Bereich der Herstellung und Handhabung von Lebensmitteln; - Tierkot; - Umgebungsproben im Bereich der Primärproduktion; fest, und zwar durch Berechnung der wahrscheinlichsten Keimzahl (en: most probable number, MPN). Das Verfahren beruht auf einer Miniaturisierung der Schritte der Verdünnung, Voranreicherung und selektiven Anreicherung. Das selektive Anreicherungsmedium, modifiziertes halbfestes Medium nach Rappaport-Vassiliadis (MSRV), ist für den Nachweis von beweglichen Salmonellen vorgesehen. Es ist nicht für den Nachweis von unbeweglichen Salmonellen geeignet. Es ist möglich, dass dieses Verfahren weniger für Salmonella ser. Typhi und Samonella ser. Paratyphi geeignet ist. Das Verfahren ist nicht für die Zählung von Salmonella spp. in (sehr) gering kontaminierten Proben ( 1 KBE/g) geeignet. ANMERKUNG Bei den meisten der für dieses Verfahren relevanten Matrices ist im Allgemeinen die Anzahl der unbeweglichen Salmonellen gering. In dieser Anmerkung sind Beispiele für Proben aus der Primärproduktion genannt. Die unbeweglichen Salmonella-Biovaren von Salmonella Gallinarum (Salmonella Gallinarum biovar gallinarum und Salmonella Gallinarum biovar pullorum) scheinen in Umgebungsproben nicht lange zu überleben und werden deshalb in Kot- oder Umgebungsproben (wie z. B. Staubproben) selten nachgewiesen (unabhängig vom Verfahren). Die Anzahl von anderen unbeweglichen Salmonella-Serovare in Kotproben scheint gewöhnlich gering zu sein. Zum Beispiel waren in einer Studie [4], in der etwa 1 000 Kotproben aus Legehennenbeständen und etwa 900 Kotproben aus Jungmastgeflügelbeständen analysiert wurden, weniger als 1 % der Gesamtanzahl der Proben in einer selektiven Bouillon positiv und auf MSRV-Medium gleichzeitig negativ (und vermutlich unbeweglich). Ähnliche Ergebnisse wurden in einer niederländischen Studie mit etwa 3 200 Kotproben von Schweinen erhalten (unveröffentlichte Daten). Andererseits wären im Fall der Studie in Literaturhinweis [4] fast 40 % der positiven Proben nicht nachgewiesen worden (d. h. falsch-negative Proben), wenn nur eine selektive Bouillon (in diesem Fall nach Rappaport-Vassiliadis) anstelle eines halbfesten Mediums verwendet worden wäre.
ICS: 07.100.30-Food microbiology
CTN: CEN/TC 463 - 2648347

Otras Relaciones

Acuerdo de Viena ISO/TS 6579-2:2012

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