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ASTM E1562 - 22

Standard Guide for Conducting Acute, Chronic, and Life-Cycle Aquatic Toxicity Tests with Polychaetous Annelids

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Edition date: 2022-01-01
In Force
Available languages: English
Summary:

Keywords: acute toxicity tests; aquatic tests; Capitella capitata; chronic toxicity tests; Dinophilus gyrociliatus; estuarine environments; experimental design; exposure tests; LC50 tests; life-cycle toxicity tests; marine environments; Neanthes arenaceodentata; Ophryotrocha diadema; polychaetes; reproductive tests; saline water; static tests; toxicity; toxicology ;
Scope:

1.1 This guide covers procedures for obtaining data concerning the adverse effects of a test material added to marine and estuarine waters on certain species of polychaetes during short- or long-term continuous exposure. The polychaete species used in these tests are either field collected or from laboratory cultures and exposed to varying concentrations of a toxicant in static or static-renewal conditions. These procedures may be useful for conducting toxicity tests with other species of polychaetes, although modifications might be necessary.

1.2 Modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, the results of tests conducted using unusual procedures are not likely to be comparable to those of many other tests. Comparisons of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting acute, chronic, or life-cycle tests with other species of polychaetes.

1.3 These procedures are applicable to most chemicals, either individually or in formulations, commercial products, and known or unknown mixtures. With appropriate modifications, these procedures can be used to conduct these tests on factors such as temperature, salinity, and dissolved oxygen. These procedures can also be used to assess the toxicity of potentially toxic discharges such as municipal wastes, sediments/soils, oil drilling fluids, produced water from oil well production, and other types of industrial wastes. An LC50 (medial lethal concentration) may be calculated from the data generated in each acute and chronic toxicity test when multiple concentrations are tested. Growth, determined by a change in measured weight, and reproduction, as the change in total number of organisms, are used to measure the effect of a toxicant on life-cycle tests; data are analyzed statistically to indicate that concentration at which a significant difference occurs between the test solutions and control(s).

1.4 The results of dose-response acute or chronic toxicity tests with toxicants added experimentally to salt water should usually be reported in terms of an LC50 (mortality), or EC50 (medial effect concentration). The results of life-cycle toxicity tests with toxicants added experimentally to salt water should be reported as that concentration at which a statistically significant difference in the number of offspring or growth (determined by weight) is produced with reference to the control(s).

1.5 Where appropriate, this standard has been designed to be consistent with or complementary to other methods for assessing toxicity to invertebrates described in Test Methods E1367 and E1706, and Guides E1391, E1525, E1611, and E1688.

1.6 This guide is arranged as follows:

 

Section

Referenced Documents

 2

Terminology

 3

Summary of Guide

 4

Significance and Use

 5

Apparatus

 6

 Facilities

 6.1

 Construction Materials

 6.2

 Test Chambers

 6.3

 Cleaning

 6.4

 Acceptability

 6.5

Safety Precautions

 7

Dilution Water

 8

 Requirements

 8.1

 Source

 8.2

 Treatment

 8.3

 Characterization

 8.4

Test Material

 9

 General

 9.1

 Stock Solution

 9.2

 Test Concentrations

 9.3

Test Organisms

10

 Species

10.1

 Age

10.2

 Source

10.3

 Feeding

10.4

 Holding

10.5

 Quality

10.6

Procedure

11

 Experimental Design

11.1

  Acute Test

11.1.1

  Chronic Test

11.1.2

  Life-Cycle Test

11.1.3

 Test Condition Specifications

11.2

  Dissolved Oxygen

11.2.1

  Temperature

11.2.2

  Loading

11.2.3

  Salinity

11.2.4

  Light

11.2.5

 Beginning the Test

11.3

 Feeding

11.4

 Duration of Test

11.5

 Biological Data

11.6

 Other Measurements

11.7

Hazards

 

Analytical Methodology

13

Acceptability of Test

14

Calculation of Results

15

Report

16

Keywords

17

Appendixes:

 

 Neanthes arenaceodentata

Appendix X1

 Capitella capitata

Appendix X2

 Ophryotrocha diadema

Appendix X3

 Dinophilus gyrociliatus

Appendix X4

1.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in Section 7.

1.9 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
ICS: 07.100.20-Microbiology of water
CTN: E50.47 - E50.47

Standards Cancellations

Anula a E1562-00R13

Otras Relaciones

Es referenciada por E1850-04R19

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