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ASTM E1295 - 22

Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with Ceriodaphnia dubia

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Edition date: 2022-01-01
In Force
Available languages: English
Summary:

Keywords: Ceriodaphnia dubia; Cladocera; effluent; life cycle; test material; three-brood toxicity test;
Scope:

1.1 This guide describes procedures for obtaining data concerning the adverse effects of an effluent or a test material (added to dilution water, but not to food) on Ceriodaphnia dubia Richard 1894, during continuous exposure throughout a portion of the organism's life. These procedures should also be useful for conducting life cycle toxicity tests with other Cladocera (Guide E1193), although modifications will be necessary.

1.2 These procedures are applicable to most chemicals, either individually or in formulations, commercial products, or known mixtures, that can be measured accurately at the necessary concentrations in water. With appropriate modifications these procedures can be used to conduct tests on temperature, dissolved oxygen, pH, dissolved ions, and on such materials as aqueous effluents (see also Guide E1192), leachates, oils, particulate matter, sediments (see also Guide E1706), and surface waters. Renewal tests might not be applicable to materials that have high oxygen demand, are highly volatile, are rapidly biologically or chemically transformed, or sorb to test chambers. If the concentration of dissolved oxygen falls below 4 mg/L or the concentration of test material decreases by more than 20 % in test solution(s) at any concentration between renewals, more frequent renewals might be necessary.

1.3 Other modifications of these procedures might be justified by special needs or circumstances. Results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparisons of results obtained using modified and unmodified versions of these procedures might provide useful information on new concepts and procedures for conducting three-brood toxicity tests with C. dubia.

1.4 This guide is arranged as follows:

 

Section

 

Referenced Documents

2

Terminology

3

Summary of Guide

4

Significance and Use

5

Apparatus

6

 Facilities

6.1

 Construction Materials

6.2

 Test Chambers

6.3

 Cleaning

6.4

Reagents and Materials

7

Hazards

8

Dilution Water

9

 Requirements

9.1

 Source

9.2

 Treatment

9.3

 Characterization

9.4

Test Material

10

 General

10.1

 Stock Solution

10.2

 Effluent

10.3

 Test Concentration(s)

10.4

 Collection

10.5

 Sample Containers

10.6

 Preservation

10.7

 Treatment

10.8

Test Organisms

11

 Species

11.1

 Age

11.2

 Source

11.3

 Brood Stock

11.4

 Food

11.5

 Handling

11.6

 Quality

11.7

Procedure

12

 Demonstration of Feasibility

12.1

 Experimental Design

12.2

 Dissolved Oxygen

12.3

 Temperature

12.4

 Preparing Test Solutions

12.5

 Conditioning Test Chambers

12.6

 Beginning a Test

12.7

 Renewing Test Solutions

12.8

 Duration of Test

12.9

 Biological Data

12.10

 Other Measurements

12.11

 Test Material

12.12

Analytical Methodology

13

Acceptability of Test

14

Calculation

15

Report

16

Appendixes

 

 Food

Appendix X1

 Culture Techniques

Appendix X2

 Test Chambers

Appendix X3

 Statistical Guidance

Appendix X4

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 8.

1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
ICS: 07.100.20-Microbiology of water
CTN: E50.47 - E50.47

Standards Cancellations

Anula a E1295-01R13

Otras Relaciones

Es referenciada por E1022-22

Es referenciada por E2172-22

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